Role of ERK in Modulating MMP 2 and MMP 9 with Respect to Tumour Invasiveness in Human Cancer Cell Line MCF-7 and MDA-MB-231

Abstract

Extracellular signal regulated kinase (ERK) is a key molecule located in the Mitogen activated protein kinase (MAPK) pathway responsible for several cell process in cancer including cell invasiveness which primarily involved breakdown of extracellular matrix. Matrix metalloproteinase facilities cell invasiveness by degrading Gelatinase which are again differentially regulated by Integrins, a family of receptors. Our study analyses the role of ERK in modulating cell invasiveness. Aims: the role of ERK1/2 in modulating MMP2 and MMP9 and thereby cell migration and invasion. Materials & Methods: The study involves downregulation of ERK through potent inhibitors and ERK1/2 siRNA and its effect on MMP2 and MMP9 and Integrin alpha 5 and beta 1 molecules. Western Blot, Gelatin Zymography, Cell invasion and Cell Migration assays are the primarily used to interpret the effect. Result: There was a considerable change in MMP 9 and MMP 2 after ERK inhibition which was also reflected in the downstream process of the ERK pathway and Integrins alpha 5 and Beta 1. Conclusion: There might be a direct correlation of ERK activity and MMP2 and MMP 9 activity with targeted modulation of ERK. Specific inhibition of ERK also might lead to integrin regulation.