Antioxidant and Neuroprotective Efficacy of Hydroalcoholic Extracts of Pomegranate Peel

Satish Kumar, Girish Chandran, R P Singh


Pomegranate (Punica granatum) peel was extracted with water: ethanol (1:1) at room temperature and the extract was evaluated for antioxidant potential against AAPH induced lipid peroxidation in vitro in mice synaptosomes. In addition, its neuroprotective efficacy against rotenone-induced mortality, motor deficits and oxidative stress in Drosophila melanogaster was also assessed. Extract showed high radical scavenging activity (RSA) as determined by DPPH and ABTS methods. Reconstituted aqueous extract showed inhibitory effect against AAPH induced lipid peroxidation in rat brain homogenate and synaptosomes. The extract also showed inhibition of acetycholinestrase (AChE) activity in the brain homogenate (IC50 =56.2µg/ml). The extract provided significant protection against rotenone, as determined by percentage mortality and escape tests. The experiments conducted on head and body of the flies indicate the protection offered by extracts, as determined by content of thiols and hydroperoxides and the levels of Catalase, superoxide dismutase (SOD), thioredoxin reductase (TRx), glutathione-S-transferase (GST) and acetylcholine esterase (AchE).


Pomegranate; DPPH; AAPH; Synaptosomes; Acetylcholinesterase; Drosophila melanogaster

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