Protein Methylation and Demethylation in Cancer
Young
Zoon Kim
Young
Zoon Kim, M.D., Ph.D, Division of Neuro-Oncology and Department of
Neurosurgery, Samsung Changwon Hospital, Sungkyunkwan University School of
Medicine, Changwon, South Korea
Correspondence to: Young Zoon Kim, MD, PhD, Division of
Neurooncology and Department of Neurosurgery, Samsung Changwon Hospital,
Samsung Medical Center, Sungkyunkwan University School of Medicine, 158
Paryong-ro, Masanhoewon-gu, Changwon, Gyeongsangnam-do, 630-723, South Korea
Email: yzkim@skku.edu
Telephone: +82-55-2906561
Fax:
+82-55-2906899
Received: February 27,
2015
Revised: July 16, 2015
Accepted: July 21, 2015
Published online: September 1, 2015
ABSTRACT
Epigenetic
modifications are heritable chromatin alterations that contribute to the
temporal and spatial interpretation of the genome. The epigenetic information
is conveyed through a multitude of chemical modifications, including DNA
methylation, reversible modifications of histones, and ATP-dependent
nucleosomal remodeling. Deregulation of the epigenetic machinery contributes to
the development of several pathologies, including cancer. During the last
decade, we saw an explosion of studies investigating the role of protein
methylation/demethylation of histones. Protein methylation and demethylation
are catalyzed by protein methyltransferases and protein demethylases. Their
substrates have been shown to play important roles in cancers. Although the
underlying mechanisms of tumorigenesis are still largely unknown, growing
evidence is starting to link aberrant regulation of methylation to
tumorigenesis. This review focuses on summarizing the recent progress in
understanding of the function of protein lysine and arginine
methylation/demethylation. We also discuss the potential and the caveats of
targeting protein methylation for the treatment of cancer.
© 2015 ACT. All
rights reserved.
Key words: Epigenetics; Neoplasm; Histone modification; Protein
methylase; Protein demethylase
Kim YZ. Protein
Methylation and Demethylation in Cancer. International
Journal of Neurology Research 2015; 1(3): 129-140 Available from: URL:
http://www.ghrnet.org/index.php/ijnr/article/view/1089
INTRODUCTIONS
Almost every nucleated cell in an organism contains
the identical complement of genomic DNA, yet different cell types express and
repress different arrays of genes as the basis for tissue and organ
differentiation. Whether a specific gene is transcribed or repressed is
determined by the conformational state of the chromosomal DNA–protein complex
referred to as chromatin, and by the ability to recruit transcription factors
and allied proteins to specific promoter sites[1]. Both chromatin
conformation and transcription factor recruitment are controlled by epigenetic
enzyme-mediated covalent modification of the DNA and the protein components of
chromatin. Genomic DNA is modified by methylation of the C5 position of
cytosine within CpG dinucleotide sites of genes by the DNA methyltransferase
(DNMT) class of enzymes[2]. The structural proteins of chromatin are
referred to as histones; there are a number of posttranslational modifications
of histones which are mediated by specific enzymes that impact chromatin conformation
and transcription factor recruitment[3]. Specific amino acid
residues of histones are modified by covalent attachment of methyl groups,
acetyl groups, phosphate groups and ubiquitin. These groups can be added and
removed by specific classes of enzymes: protein methyltransferases (PMTs);
protein demethylases (PDMs); histone acetyl transferases (HATs); histone
deacetylases (HDACs); protein kinases; protein phosphatases; protein ubiquitin
ligases (E3s) and protein ubiquitin carboxyl-terminal hydrolases (UCHs).
During the
last decade, we saw an explosion of studies investigating the role of protein
methylation/demethylation of histones. ‘Ying-Yang’ posttranslational
modifications are important to fine-tuning the activity of these histone
proteins. Protein methylation and demethylation are catalyzed by protein
methyltransferases and protein demethylases. Their substrates have been shown
to play important roles in cancers. Although the underlying mechanisms of
tumorigenesis are still largely unknown, growing evidence is starting to link
aberrant regulation of methylation to tumorigenesis (Table 1). In this review,
we aim to summarize the recent progress in understanding of the function of
protein lysine and arginine methylation/demethylation. We also describe the
potential and the caveats of targeting protein methylation for the treatment of
cancer.
PROTEIN LYSINE METHYLATION
Protein lysine methylation has gained tremendous attention since the
discovery of SUV39H1 as the first histone lysine methyltransferase in 2000[4].
Following the discovery, numerous proteins have been found to possess
methyltransferase activity, such as G9a/GLP[5,6], MLLs[7],
EZH2[8], SET2[9], SET7/9[10], DOT1[11,12],
and PR-SET7 (also known as SETD8)[13]. These enzymes catalyze the
transfer of methyl group from the co-factor S-adenosyl-L-methionine (SAM) to
the lysine residues of histones. More recently, many non-histone proteins have
been identified as substrates for these enzymes, hence the name protein lysine
(K) methyltransferases (PKMTs) (Table 2). It is worth pointing out that PKMTs
play important roles in other biological processes including developmental
biology and stem cell differentiation. However, here we will focus on the
implications of PKMTs in cancers, especially.
Methylation of lysines residues is
known to occur usually on histone H3 (K4, K9 and K27) and H4 (K20). As
mentioned above, the SUV39 protein was the first histone methyltransferase to
be discovered[4]. The methyltransferase activity of SUV39 is
directed against lysine 9 of histone H3 and its catalytic domain resides within
a highly conserved structure, the SET domain. The sequences within the SET
domain are not however sufficient for enzymatic activity. Methylation is only
seen when two flanking cystein-rich sequences (PRE-SET and POST-SET) are fused
to the SET domain. Use of the simple modular architectural research tool
(SMART) indicates that there are 73 entries in the human database which possess
a SET domain. In contrast, there are 6 SET domain proteins in Saccharomyces
cerevisiae, 11 in Schizosaccharomyces pombe, 41 in Drosophila and
37 in Caenorhabditis elegans. Previously characterized human proteins
possess a SET domain showing that they can be grouped into four classes (Figure
1). The classification is based on the similarity between the human SET domains
as primary and their relationship to SET domains in yeast (S. cerevisiae)
as secondary. Two groupings show similarity to either yeast SET1 or SET2, thus
defining two of the classes. Another class has SUV39 as its defining member and
a fourth family represents homologues of the RIZ SET domain. The four families
described may subdivide to further classes when more information is available.
Overall, the subdivisions indicate that enzymes with sequence similarity in
their SET domain also have other structural features (i.e. domains) in common.
Figure 2 illustrates protein lysine methylation/demethylation and their
biological roles in terms of transcriptional activity, Figure 3 summaries
methylase and demethylase at the major 6 lysines of histone tail according to
genetic regulatory activity. Next topic is a description of the defining
features of each family emphasizing wherever possible, their links to chromatin
and transcriptional regulation.
The SET1 family
Two uncharacterized homologous human proteins, designated here as
hSET1A and hSET1B are highly related in their SET domain to yeast SET1 (ySET1)
that has the capacity to methylate lysine 4 of histone H3. Other related members
in this family include proteins of the polycomb and trithorax (trx) group of
genes, whose function is linked to the epigenetic regulation of regulated genes
in development. They include the polycomb proteins EZH1 and EZH2 (also known as
ENX1 and ENX2), the TRX proteins MLL (also known as TRX1, HRX, ALL1, HTRX),
MLL2 (also known as TRX2, HRX2) and the related protein ALR. A defining
structural feature of this family is a SET domain at the very carboxyl terminus
of the protein that is mostly followed by a POST-SET domain. The two proteins
that do not fit this bill are EZH1 and EZH2. These have no POST-SET domain and
may therefore represent a subset of this family, or a distinct family.
EZH2 is one of the first PKMTs
implicated in human cancers[14,15]. Its expression is highly
correlated with the metastasis of various cancers, such as prostate and breast
cancers. EZH2 is the enzymatic subunit of polycomb repressive group 2 (PRC2)
that methylates histone H3 at K27[16]. However, the underlying
mechanisms of oncogenic effect of EZH2 are not fully understood. It is also
unclear whether H3K27 methylation is required for the role of EZH2 in
tumorigenesis since EZH2 may have other substrates beyond histone H3[17].
The only connection to chromatin regulation comes from the fact that EZH2 is
part of a deacetylase complex[18] and it binds a protein homologous
to the ATPase SNF2[19]. The TRX-related MLL proteins on the other
hand have been studied extensively, mainly as a consequence of their link to
cancer[20]. Many translocations have been found between MLL and
other proteins that play a causative role in leukemia. The most notable
translocations (in a chromatin context) are fusions of MLL to the CBP acetylase
and to AF10, a protein whose Drosophila homologue associates with HP1[21].
Interestingly, MLL has a bromo-domain (an acetyl-lysine binding structure), a
link which may be relevant to CBP. In addition, it has a MBD domain that may
allow it to recognize methylated DNA[22].
DOT1L performs H3K79 methylation, a modification
that is associated with transcription elongation. One of its pathological roles
is the mis-regulation of the Hox gene expression through interacting with AF9,
a fusion partner of MLL. The misregulation can lead to leukemogenesis[23].
Another
promising therapeutic target for cancer is SMYD2. A recent paper has shown that SMYD2 is involved in
maintaining an undifferentiated status of MLL-AF9-induced acute myeloid
leukemia (AML)[24]. Although the mechanism underlying this leukemia
maintenance is unclear, SMYD2 has been shown to methylate p53[25]
and Rb[26] which are the most important tumor suppressors. In
addition, SMYD2 is reportedly overexpressed in esophageal squamous cell
carcinoma[27]. Knockout mice for SMYD2 have been generated[28].
Future work needs to address whether SMYD2 knockout mice are resistant to
tumorigenesis in response to oncogenic insults at various tissues. In addition,
the epigenetic role of SMYD2 in cells is largely unknown. One report showed
that SMYD2 methylates histone H3K36[29], while another report
suggested that SMYD2 is an H3K4 methyltransferase[30]. These
observations merit further studies to elucidate the mechanisms underlying this
dual-substrate specificity.
The SUV39
Family
The most striking feature in this group of proteins is the presence of
a PRE-SET domain in all members, and indeed the absence of such a domain in any
protein outside the SUV39 family. Given the necessity of this domain for
enzymatic activity[4], it is suggested that this domain may provide
specificity necessary for the SET domain to methylate lysine 9 of histone H3,
rather than any other lysine. Consistent with this idea, three members of this
family (in addition to SUV39H1) have been identified as histone H3 lysine 9
methyltransferases: SUV39H2[31], G9A[32] and ESET[11].
The chromo-domain present in SUV39 is perhaps surprisingly not present in any
other SET domain protein. However, the function of this domain is still
unclear. Although it does not bind methylated lysine 9, (in contrast to the HP1
chromo-domain), it is still possible that it may recognize other methylated
lysines in histones or in other proteins.
SUV39H1 and its homolog
SUV39H2 are required for heterochromatin formation. Double knockout of SUV39h1
and SUV39h2 mice are subject to genomic instability[33].
SUV39h1-dependent senescence has been shown to protect mice from Ras-driven
invasive T-cell lymphoma[34]. Based on these studies, SUV39H1
appears to play a tumor-suppressive function. Controversially, SUV39H1-mediated
H3K9me has been linked to gene silencing of the tumor suppressor genes, such as
p15INK4B and E-cadherin, in AML[35]. Therefore, it is highly possible
that the default function of SUV39H1 is to maintain genome stability by
limiting the acute activation of oncogenes while its dysregulation could cause
tumor formation.
G9a and GLP belong to one new group of methyltransferases that
methylate p53. They have a wide range of biological and pathological functions.
From the cancer perspective, G9a and GLP regulate the apoptotic function of
p53. The di-methylation of p53-K373 by G9a and GLP decreases the
transcriptional activity of p53. Interrogation of Oncomine database reveals
that G9a is overexpressed in various tumors, further suggesting its oncogenic
effects. Indeed, the overexpression of G9a was shown to increase metastasis and
invasion in lung cancer[36]. However, the ultimate outcome of
inhibition of G9a/GLP could be complicated by the fact that they methylate
other histone and non-histone substrates[37]. G9a and GLP are
largely responsible for H3K9 mono- and di-methylation. Recently, a
distinguishing feature of cancer cell lines, i.e. the loss of G9a-dependent
large block of H3K9me2, was observed[38]. This suggests that the
loss of G9a activity or its substrate H3K9me2 confers growth or survival
advantage to cancer cells. Therefore, whether the inhibition of G9a/GLP can
decrease the growth or increase the apoptosis of cancer cells requires further
investigation.
Two proteins, ESET and CLLL8 have what appears to be an expanded SET (ESET)
domain. Close analysis reveals that this is as a result of a large insertion of
sequence (>300 residues for ESET and >170 for CLLL8) between two highly
conserved blocks within the SET domain. In all other proteins, these two sub
domains are found directly adjacent to each other. This insertion evidently
does not seem to affect either enzymatic activity or specificity. One possible
scenario is that this sequence forms a ‘hinge domain’ that may regulate
activity of the enzyme following the binding of proteins. Interestingly, both
ESET and CLLL8 contain a MBD domain, a structure that is potentially capable of
recognizing methylated DNA[39]. This structure may be able to direct
the lysine methylase activity of ESET to DNA methylated promoters.
LYSINE DEMETHYLATION
Like other protein modifications, lysine methylation is also subject to
its counter modification, demethylation (Table 2). For histones, the first
reported demethylase is lysine-specific demethylase 1 (LSD1, also known as
BHC110)[40,41]. However, LSD1 can only demethylate mono- or
di-methylated lysines. Shortly after the discovery of LSD1, a second family of
enzymes, Jmj C-domain containing proteins, was found to have demethylation
activity for tri-methylated, as well as mono- and di-methylated lysines[42].
These enzymes are referred to as protein lysine demethylases (PKDMs). The roles
for PKDMs in human diseases, including cancer and neurological disorders, are
beginning to be delineated[43,44]. The clinical relevance of
demethylase inhibitors has not been demonstrated using a small molecule;
however, a few interesting inhibitors have been disclosed. Demethylases of the
LSD1/KDM1 family share some sequence and structural similarities to amine
oxidases[45] and monoamine oxidase. Inhibitors, such as
tranylcypromine, have been shown to inhibit LSD 1 by forming a covalent adducts
between the flavin cofactor and the inhibitor[46]. The Jmj C-domain
containing demethylase (JHDM) family conforms to a different catalytic
mechanism, relying on an active site iron and a 2-oxoglutarate cofactor.
Analogs of the 2-oxoglutarate cofactor have been shown to be inhibitors of recombinant
enzyme and to increase methylation in cell systems[47].
The Amine
Oxidase Family
LSD1 may serve as a
viable target for therapeutic intervention in cancers. It decreases the
activity of p53. However, recent studies have discovered some controversial
roles of LSD1. LSD1 has been shown to demethylate p53 and decrease the
apoptotic effect of p53, suggesting that it can act as an oncogene. Indeed, the
overexpression of LSD1 is observed in prostate cancer[48] and also
correlates with poor prognosis of neuroblastoma[49]. Several reports
have also shown that LSD1 has a potential role in the repression of E-cadherin,
a molecule mediating the cell–cell junction, and cell migration[50-52].
The expression of E-cadherin is inversely correlated with metastasis. All of
these studies suggest that LSD1 is a putative onco-protein. However, one report
proposes that LSD1 can suppress the metastasis of breast cancer by repressing
tumor growth factor (TGF)-beta1 signaling[53]. Unfortunately, the
exact cause of this controversy is at present unknown. It is possible that the
function of LSD1 is regulated by other binding partners and the ultimate effect
is context-dependent. LSD2, the homolog of LSD1, was recently shown to have
demethylation activity toward H3K4me2[54]. So far, there is no
report to indicate that LSD2 plays a role in tumorigenesis.
The Jmj-domain
Family
JMJD2c (also known as
GASC1) is a member of the Jmj C-domain containing protein family. The members
of this family, as described above, can demethylate mono-, di- and/or
tri-methylated lysines. JMJD2c was characterized as an H3K9me3/me2 demethylase.
The overexpression of JMJD2c was observed in esophageal squamous carcinoma[55].
It regulates androgen receptor-mediated gene expression[56].
Therefore, it could also play an important role in androgen receptor-dependent
prostate cancer, although this hypothesis needs to be tested formally. Very
recently, functional interplay between JMJD2c and JAK2, a histone tyrosine
kinase[57], has been revealed in B cell lymphoma and Hodgkin’s
lymphoma[58]. These findings provide a mechanistic rationale for
testing the co-inhibition of JAK2 and JMJD2c in cancers. It is important that
JMJD2c and LSD1 also should cooperate in androgen receptor-regulated gene
expression[56]. These observations fuel the idea of investigating
the effects of LSD1 and JMJD2c inhibition in prostate cancer.
The RIZ family
The RIZ (retinoblastoma-interacting zinc finger) protein is the most
characterized member of this eponymous family[59] that contains two
other proteins, BLIMP and PFM1. This is a homogeneous family in the sense that
they have very similar structural features. They all have a SET domain towards
the amino terminus, they have no PRE or POST-SET, and possess a number of
zinc-finger motifs clustered towards the carboxyl terminus. Close inspection of
the SET domain of these three proteins reveals striking sequence changes in a
highly conserved motif (NHSC) which, when mutated, abolishes catalytic activity[4].
The histidine is invariant in all other SET domains and may represent a
catalytic residue. In fact, they are not protein lysine demethylase. However,
the changes in this motif in members of the RIZ family suggests that they may
possess an altered substrate specificity or indeed may not possess
methyltransferase activity and may, therefore, function as antagonists for the
active enzymes. The ability of some SET domains to bind histones[60]
may be relevant to this point.
Current
information concerning the RIZ family suggests that they are transcriptional
regulators controlling differentiation and that they may be involved in cancer.
The RIZ protein can bind and stimulate the activity of estrogen receptors[61]
and can repress the activity of SP1-site containing promoters[62].
Both of these functions rely on an intact SET domain. There are two spliced
variants of RIZ one of which, RIZ1, contains the SET domain, whereas the other,
RIZ2, does not. Mice lacking RIZ1 develop diffuse large B-cell lymphomas and
other tumours. Given that RIZ2 is still expressed in these mice, a role of the
SET domain in tumor predisposition is suggested.61 The BLIMP1 protein (B-cell
induced maturation protein 1) is a transcriptional repressor implicated in the
silencing of the c-myc, IFN-β and CIITA genes[63-65]. Little is
known about PFM1, but its profile suggests that it is involved in
differentiation. Like RIZ, it has alternate mRNA products, one of which
probably lacks a SET domain, and maps to a chromosomal location frequently
deleted in tumours[66].
ARGININE METHYLATION
Apart from lysine methylation, arginine (R) methylation has been also
known to play certain roles in cancer (Table 3). The history of arginine
methylation was recently surveyed[67]. Several arginine residues are
also modified by methylation. These include, R2, R8, R17, and R26 of histone
H3, and R3 of histone H4. Arginine residues may undergo mono-methylation,
symmetric di-methylation, or asymmetric di-methylation. There are five known
arginine methyltransferases that have a highly conserved catalytic domain.
PRMT1, PRMT3 and PRMT4/CARM1 are classified as Class I enzymes as they can
catalyze the formation of asymmetric di-methylated arginine whereas PRMT5/JBP1
is classified as a class II enzyme as it catalyzes symmetric di-methylation.
The PRMT2 protein has not yet been established as an enzyme[68].
H3R2 is asymmetrically di-methylated by CARM1/PRMT4[69] and PRMT6[70].
H3R8 is methylated by PRMT5[71], while H3R17 and H3R26 are
asymmetrically di-methylated by CARM1/PRMT4, which also methylates H3R2[69].
Finally, H4R3 is mono-methylated by PRMT172 and di-methylated, both
symmetrically and asymmetrically by PRMT5[73]. The methylation of
specific arginine residues contributes to the regulation of cell fate. For
example, ectopic expression of CARM1 in mouse blastomers increases the levels
of arginine methylation and promotes the dramatic upregulation of the
pluripotency genes NANOG and SOX2. This, in turn, promotes the cycling of
pluripotent cells and the expansion of the inner cell mass of the blastocyst[74].
The molecular
mechanisms by which arginine methylation contributes to chromatin structure and
transcriptional regulation are not yet clear. However, it has been shown that
arginine methylation may regulate the modification or recognition of
neighboring histone residues. Thus, it has been shown that methylation of H3R2
prevents the tri-methylation of H3K4 and vice versa[70].
Furthermore, it has been shown that asymmetric methylation of H3R2 inhibits the
association of the TFIID subunit TAF3 with H3K4me3[75].
Arginine
methylation of histones
Methylation of histones by arginine methyltransferases has been known
for some time, but only recently has this modification been validated as a
functional event because the methylation of arginine on histones was not easily
detectable by standard bulk-histone sequencing. A breakthrough came when CARM1
(now known also as PRMT4) was isolated as a two-hybrid interacting partner of
GRIP1, a p160 family co-activator of nuclear hormone receptors[76].
CARM1/PRMT4 acted as a co-activator of nuclear receptor activity using an
arginine methyltransferase domain capable of methylating specifically histone
H3 in vitro. This was the first clue that methylation of histones at arginine
may be a stimulating event for transcription. Confirmation of this has come
recently using antibodies that specifically recognize the major methylation
site by CARM1, Arg17 of histone H3. These antibodies, when used in chromatin
immunoprecipitation assays, identify the presence of a methyl group on Arg17
only when nuclear receptor regulated promoters are active[77,78].
Arginine methylation thus represents a histone modification that correlates
with the active state of transcription, much like acetylation. Interestingly,
CARM1/PRMT5 co-operates with the p300 acetylase to stimulate transcription by
nuclear receptors[79], raising the possibility of cross talk between
methylation and acetylation.
The PRMT1
methyltransferase has also been shown to mediate methylation of histones in vivo.
Antibodies raised against methylated Arg3 of H4 (the site of PRMT1 methylation)
recognizes bulk-purified histones[72,80]. That PRMT1 is the major
Arg3 methyltransferase is established from the fact that Arg3 is not methylated
in PRMT1–/– cell lines. Target promoters at which Arg3 of H4 is methylated have
not yet been identified. However, a good candidate would be nuclear receptor
stimulated genes as PRMT1 is found associated with p160 co-activators and can
co-operate with CARM1/PRMT4 to stimulate such genes[81]. As with
PRMT4/CARM1, methylation by PRMT1 has been shown to have a functional
connection to acetylation on histone H4. Wang et al[72]
showed that methylation of Arg3 can augment the subsequent acetylation of H4,
indicating once again the cross talk between two stimulating modifications.
Arginine
methylation within non-histone proteins
Although this review has a focus on methylation of histones no review
on arginine methyltransferases can be complete without a mention of non-histone
methylated proteins, since these have been the primary focus of research since
the discovery of methylation over thirty years ago. These studies may give
clues to the function of histone methylation. Well-studied substrates include
heterogeneous nuclear ribonucleoproteins hnRNPs, which are involved in pre-mRNA
splicing and RNA transport. Methylation in these proteins can occur at RGG. In
the case of a yeast hnRNP, Np13p, methylation affects its nuclear-cytoplasmic
localization[82,83]. Other methylated substrates include snRNPs
(SmD1 and SmD3) which are methylated at GRG motifs[84] and myelin
basic protein[68].
Methylation of
two non-histone proteins links arginine methylation to signal transduction
pathways. The first is Sam68, a substrate for
the Src kinase, whose methylation within a proline rich motif leads to
diminished binding to SH3 containing signaling proteins[85]. The
second substrate is STAT1, a transcription
factor involved in interferon-induced signal transduction. Methylation of STAT1
takes place on Arg 31and is mediated by PRMT1. This methylation augments the
transcriptional activation capacity of STAT1, at least partly, by inhibiting
its interaction with a negative regulator PIAS1[86]. A modification
of the CBP/p300 acetylase was recently discovered which directly links arginine
methylation to transcription regulation via a non-histone protein[87].
The CARM1 protein methylates CBP/p300 near the KIX domain which is necessary
for the insertion for CBP/p300 with the CREB transcription factor. The net
result is that methylation prevents activation by CREB through displacing
CBP/p300.
PROTEIN METHYLATION AS
IMPLICATED TARGETS IN CANCER
The central role of epigenetic abnormalities in tumor evolution has
prompted efforts for the development of therapeutic approaches that aim to
reverse epigenetic changes. The cytosine analogs 5-azacytosine (azacytidine) and
2-deoxy-5-azacytidine (decitabine) are currently the most advanced drugs for
epigenetic cancer therapies. These DNA methyltransferase inhibitors have shown
remarkable efficiency in the derepression of epigenetically silenced tumor
suppressor genes[88,89]. Here we will focus on the clinical
implications and the recent progress in the discovery of selective PKMT
antagonists in cancers. It is worth pointing out that PKMTs play important
roles in other biological processes including developmental biology and stem
cell differentiation.
Antagonist of
protein lysine methyltransferase
Since Greiner et al[90] discovered the first
selective, non-nucleoside inhibitor of recombinant Drosophila
Su(var)3–9, chaetocin in 2005, the
antagonist discovery effort has quickly gained momentum and a number of new
antagonists have emerged. Herein, we describe selective PKMT antagonists with
an emphasis on most recently discovered compounds. PRMT antagonists were
recently surveyed and are not included in this review[91]. Chaetocin was found to
antagonize SUV39H1, the human ortholog of Drosophila Su(var)3–9, as well
as other H3K9 PKMTs, including Neurospora DIM5 and mouse G9a. It was
selective over non-H3K9 PKMTs, such as H3K27 PKMT dE(z) complex, H3K4 PKMT
SET7/9, and H4K20 PKMT SETD8[90]. Interestingly, a total synthesis
report found natural (+)-and synthetic (-)-chaetocin to be equipotent against G9a[92].
In addition, it was found that chaetocin inhibited thioredoxin reductase[93]. Like other members of
the epidithiodiketopiperazine class[94], chaetocin is cytotoxic.
Despite its cytotoxicity, it was reported that chaetocin-treated Drosophila
SL-2 cells at an antagonist concentration of 0.5 μM showed marked reduction in
cellular levels of di- and tri-methylated H3K9 without apparent changes in
cellular levels of methylated lysines (such as H3K27, H3K36, H3K79, and H3K4)[90].
Discovery of BIX01294, the first
selective small molecule inhibitor for G9a and GLP, by Jenuwein and co-workers[95]
was an important advance in the PKMT antagonists’ discovery field, as this
compound was the first PKMT antagonist that blocks protein–protein interactions[96].
BIX01294 had good in
vitro potency against G9a and GLP and was selective over other H3K9 PKMTs
(SUV39H1 and SETDB1), H3K4 PKMT SET7/9[96]. Unfortunately BIX01294 was toxic in
cellular assays at concentrations above 4.1 μM. Mechanistically, unlike
chaetocin, BIX01294 did not inhibit
G9a in a SAM-competitive manner but rather occupied the histone peptide binding
pocket, which was evidenced by the X-ray crystal structure of BIX01294 and GLP
in the presence of SAH (S-adenosyl-L-homocysteine)[95,96]. This
crystal structure revealed that BIX01294 did not bind in the SAM-binding
site nor did it interact with the lysine binding channel[96].
By elaborating
the 7-methoxy moiety of the quinazoline template, it was discovered that a
series of new analogs interacted with the lysine-binding channel, including UNC0224, a 7-fold more
potent G9a inhibitor in the G9a ThioGlo assay when compared with BIX01294[97,98].
A high-resolution (1.7Å) X-ray co-crystal structure of G9a and UNC0224 (PDB:
3K5K) showed that the 7-dimethylamino propoxy side chain of UNC0224 only
partially occupied the lysine binding channel of G9a[95,98], and
thus space remained to accommodate a longer side chain or larger aminocapping
group.
The most
potent G9a antagonist to date, UNC0321 was a result of further side chain manipulations[99].
Because UNC0321 likely reached
the detection limits of the biochemical assays, Morrison Ki values were
determined using an endoproteinase-coupled microfluidic capillary
electrophoresis assay[100]. UNC0321 (Morrison Ki = 63 pM) was about
40-fold more potent than UNC0224 (Morrison Ki = 2.6 nM) and 250-fold more
potent than BIX01294 (Morrison Ki = 16 nM)[99]. While highly potent
in biochemical assays, UNC0321 was less potent
in cellular assays in comparison with BIX01294, prompting the development of
analogs with higher cellular potency.
UNC0638, specifically
designed to increase lipophilicity and cell membrane permeability while
maintaining high in vitro potency, was indeed found to have excellent in vitro
potency (Morrison Ki G9a = 3.7 nM; Ki = 3.0 nM) and was > 100-fold selective
over a wide range of epigenetic and non-epigenetic targets[101].
Mechanism of action studies revealed that UNC0638 was competitive with the peptide
substrate and non-competitive with the co-factor SAM. The MOA findings were
confirmed by X-ray crystal structure of the G9a–UNC0638– SAH complex (2.56Å
resolution; PDB: 3RJW). The combination of high potency, excellent selectivity,
low cell toxicity and robust on-target activities in cells makes UNC0638 an excellent
chemical probe of G9a/GLP for cellular studies. Most recently, UNC0646 and UNC0631, close analogs
of UNC0638, were reported to have comparable cellular potency and toxicity and
could serve as alternative tool compounds for investigating specific cellular
systems[102]. For example, UNC0646 had an outstanding
toxicity/function ratio in MCF7 (470), 22RV1 (510), and IMR90 (360) cells,
making this compound potentially more useful for studying G9a biology in these
specific cell lines[102].
Chang et al[103]
also developed a potent G9a and GLP antagonist E72 based on the quinazoline template with binding
affinities determined by isothermal titration calorimetry (Kd GLP = 136 nM; G9a
= 164 nM). A brief selectivity study showed E72 was inactive against SUV39H2 with no inhibition at 5
μM[103]. The X-ray co-crystal structure of the GLP–E72 complex in
the presence of SAH (2.19Å, PDB: 3MO5) showed that E72 analogous to UNC0224
with G9a occupies both the surface of the peptide binding groove and the lysine
binding channel.103 In three separate cell types, E72 was significantly less
toxic than BIX01294 at compound concentrations of 10 μM and was able to
reactivate K-ras-mediated epigenetic silencing of the Fas gene in NIH 3T3 cells[103].
Most recently,
Daigle et al[104] discovered a highly potent and selective
SAM-competitive DOT1L antagonist EPZ004777 (Ki = 0.3 nM), which is a
co-factor product (SAH) mimic. EPZ004777 was > 1000-fold selective for DOT1L
over CRM1, EHMT2, EZH1, EZH2, PRMT1s, PRMT5, PRMT8, SETD7, and WHSC1, all of
which are SAM-utilizing methyltransferases. EPZ004777 was found to selectively kill off
cells bearing MLL translocation. In addition, in vivo administrations of EPZ004777 led to extension
of survival in a mouse MLL xenograft model[104]. Subsequently, Yao
and co-workers105 showed that protecting the N6 position in SAH does not affect
binding to DOT1L, but seems to instill selectivity against CARM1, PRMT1, G9a,
and SUV39H2. The best compound in their series, compound 1 had an IC50
of 0.11 μM against DOT1L and was proposed to be capable of covalently binding
to the histone[105].
Recently
Ferguson et al[106] described a potent antagonist of SMYD2, AZ505 (Ki = 0.30 μM) identified by high
throughput screening. As seen from its crystal structure in complex with SMYD2,
AZ505 occupies the
peptide binding groove and is peptide substrate competitive. Furthermore, this
compound was shown to be > 83 fold selective for SMYD2 over a panel of PKMTs
(SMYD3, DOT1L, EZH2, GLP, G9a, and SET7/9)[106]. Given the broad
roles of SMYD2 in cancers, it will be interesting to test the effect of this
inhibitor and its analogs in cancer cells.
Antagonists of
protein lysine demethylase
Several groups have investigated polyamine analogs for their ability to
inhibit recombinant LSD1 in vitro and in vivo. Among these compounds, the best
were polyamines 2
and 3, which antagonized LSD1 activity by 85% and 82%, respectively, at 10 μM[107,108].
Subsequently, Huang et al[109]. investigated polyamines, such
as PG11150, as antagonists of LSD1. It was found that colorectal cancer cells
treated with polyamine PG11150 should display re-expression of multiple
aberrantly silenced tumor suppressor genes. In addition, PG11144 the
trans-isomer of PG11150, displays a marked decrease in tumor growth and
increases H3K4me2 levels in the mouse xenograft model, without significant
overall toxicity, when administered in combination treatments alongside a known
DNMT inhibitor 5-azacytidine. Interestingly, when used alone PG11144 displayed
antitumor activity, while polyamine 2 did not produce a similar effect without
the accompanying DNMT inhibitor. However, selectivity of these inhibitors for
LSD1 over monoamine oxidase (MAO)-A, MAO-B and the newly discovered LSD2 is yet
to be addressed.
A high degree
of homology exists between the catalytic sites of MAO-A, B, and LSD1; thus, one
might reasonably expect that many of the existing monoamine oxidase inhibitors could antagonize
LSD1. Recognizing this, researchers tested a known non-selective MAO inhibitor
PCPA (trans-2-phenylcyclopyropylamine) and indeed found it to have LSD1
inhibitory activity (Ki = 357 μM; LSD1)[110,111]. MAO and LSD
inhibition is fairly insensitive to stereochemistry as has been shown[112,113];
thus (+)-racemates of trans-tranylcypromine and its derivatives are commonly
used for testing and in the clinic[111]. Inhibition of LSD1 by
tranylcypromine has been shown to proceed via ring-opening of the cyclopropyl
moiety followed by formation of a covalent adduct with the C(4) of the FAD
co-factor. PCPA displays no apparent selectivity for LSD1 over MAO-A, or MAO-B,
which prompted a number of groups to investigate LSD1-selective derivatives
based on its core structure.
Antagonists
based on the tranylcypromine scaffold include
compound 4 (Ki=8 μM) discovered in 2008[114], S2101 (Ki=0.6 μM,
LSD1; Ki=110 μM, MAO-A; Ki=17 μM, MAO-B) in 2010[115], compound 5
(Ki=1.9 μM, LSD1; Ki=290 μM, MAO-A) in 2009[116], and compound 6
(Ki=6 μM, LSD1) in 2010[112]. These inhibitors affect histone H3K9
and H3K4 methylation levels in cells, mediated by the inhibition of
LSD1-catalyzed demethylation.
The functional
interaction between LSD1 and HDAC has been reported by Lee et al[117];
they found that LSD1 and HDAC enhanced the activity of each other. Given the
fact that HDAC inhibitors have already been approved for cutaneous T-cell
lymphoma treatment, this molecular mechanism raises an interesting hypothesis
that LSD1 and HDAC inhibitors could cooperatively inhibit tumorigenesis.
JMJD2
demethylases are antagonized by analogs of the co-factor 2-OG, including
N-oxalylglycine (NOG), pyridine decarboxylases, and the related bipyridyl
derivative 12. Other chemotypes that are also presumed to bind to the active
site Fe (II) include catechols, hydroxamic acids, and tri-carboxylic acid cycle
intermediates, such as succinate and fumarate[118-120]. Antagonist
compound 13 and 14, designed on the basis of the crystal structure of NOG in
complex with JMJD2A (PDB ID 2OQ6), are equipped with an appendage intended to
engage a large subpocket adjacent to the active site. Hamada et al.121 have
demonstrated inhibition of JMJD2A, 2C and 2D activity by 14 and its analogs in
vitro and in vivo. Importantly, only the methyl ester prodrug of 14 was active
in cellular assays presumably due to poor cell permeability of the free
acid-containing analogs. Rose et al[122] produced a crystal
structure of an analog of their best antagonist, compound 13 in complex with
JMJD2A (PDB ID 2WWJ) confirming the predicted binding mode for their
N-oxalyl-D-tyrosinyl derivatives. Compound 13 exhibited some selectivity
against prolyl hydroxylase domain-containing protein 2 (PHD2) in biochemical
assays, while 14 appeared to inhibit other Fe(II)/a-ketogluterate-dependent
oxygenases indiscriminantly[121]. Interestingly, while NOG itself
selectively inhibits PHD1/2 over JMJD2C/2A, its analog 15 is selective for
JMJD2C and JMJD2A[123]. This selectivity is presumably based on the
presence of a methylene group next to the carbonyl of the hydroxamate moiety,
and its affinity for JMJD2 is dependent on the length of the linker leading to
the tertiary amino group. Another JMJD-selective inhibitor was recently
discovered[124]. Compound 16 was designed to incorporate both a
substrate mimic and a methyllysine mimic. This inhibitor is more than 9000-fold
selective for the Jmj C-domain containing enzymes over PHDs. Its methyl ester
prodrug methylstat is cell active and may have potential for anticancer
chemotherapy[124].
A
high-throughput assay based on time-resolved fluorescence resonance energy
transfer was reported recently to screen the antagonists for LSD1 and JMJD2c[125].
Numerous antagonists for these two enzymes have been identified through the
assay. Because LSD1 and JMJD2c cooperate in regulating the gene expression in
prostate cancer, these inhibitors will be extremely valuable for testing the
synergistic effects of co-inhibition of these two enzymes in cancer. Because of
the common mechanism underlying the demethylation reaction of JmjC
domain-containing proteins, the specificity of these inhibitors needs to be
rigorously tested in the future.
CONCLUDING REMARKS
Cancer is a genetic disease. Emerging evidence, however, is challenging
this concept by showing that non-genetic heritable changes affecting chromatin
structure and gene expression may play an equally important role in the biology
of the cancer cell. Based on this evidence, tumor development and evolution
depends on the combination of genetic and epigenetic changes. Actually, the two
of courses are not independent. Thus, epigenetic silencing of genes involved in
the response to DNA damage may give rise to genetic instability and the
accumulation of genetic changes in cancer cells. Genetic changes affecting
genes involved in epigenetic regulation, on the other hand, may alter the
epigenome of the cancer cell.
In this
review, we have focused on one part of histone methylation, namely the protein
methylation and demethylation especially in terms of associated enzymes that
regulate the status of methylation of histones. However, it is clear that
epigenetic mechanisms are interdependent and integrated into the regulatory
machinery of the cell. These epigenetic regulators can be modified by the
complex signaling pathways that become deregulated in the cancer cell. Future
studies on the cancer epigenome and its regulation will redefine our
understanding of the cancer cell and may reveal new targets for therapeutic
intervention.
ACKNOLWDGEMENTS
We would like to thank MinGyu Lee Ph.D., and Sung-Hun Lee, Ph.D.,
(Department of Molecular and Cellular Oncology, The University of Texas MD
Anderson Cancer Center) for review literature.
CONFLICT OF INTERESTS
The authors have no conflicts of interest to declare.
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Peer reviewer: Lin
Wang, Professor, Computational Biomedical Center, Beijing University of Posts
and Telecommunications, Taiyueyuan 11-307, Zhichun Road 12, Haidian District,
Beijing 100191, China; Wei-Lin JIN, Associate Professor, School of Life Science
and Biotechnology, Shanghai Jiao Tong University, 800 Dong Chuan Road, Minhang,
Shanghai, 200240, China.
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