Effect of Momordica charantia on the prevention of changes in serum and hepatic metabolism of animals fed a high fat diet

Filipe Nogueira Franco, Marcos Augusto Nascimento Porto

Abstract


Excess lipids stored in adipose tissue can lead to changes in metabolism. It is known that the treatment with Momordica charantia (MC) is able to stimulate lipolysis, decrease insulin resistance, triacylglycerol concentration and body weight. Thus, the aim of this study was to investigate the effect of MC tea administration to prevent biochemical changes in the metabolism of mice fed high fat diet. For this, swiss mice were fed with a control diet (C) or a high fat diet for 4 and 8 weeks (HF). They also received water or MC tea during treatment (CP, HFP). Body weight, food and water intake were evaluated daily. After treatment, the HF and HFP animals were submitted to the Glucose Tolerance Test. All groups were euthanized and blood was collected for analysis of biochemical parameters. The epididymal, retroperitoneal and liver tissues were weighed. hepatic tissue were also used to quantification of lipids.  The results were analyzed using Student's T test (p<0.05) and expressed as mean ± SE. A progressive decrease in the accumulation of lipids in adipose tissues and in the liver, as well as in the serum levels of TAG and VLDL was observed between the HFP8 vs HFP4 groups The carbohydrate metabolism presented very expressive changes in a short time of treatment (HFP4). The data suggest that MC appears to be effective in preventing changes in metabolism, probably by preventing the increase of lipid deposits, decreasing the inflammatory process and leading to an improvement in insulin sensitivity.


Keywords


Obesity; Adipose tissue; Insulin resistance, Fatty liver; Momordica charantia

References


Bonamichi B, Parente EB, Santos RB, Beltzhoover R, Lee J, Salles JEN. The Challenge of Obesity: A Review of Approved Drugs and New. Journal of Obesity & Eating Disorders 2018; 4(1):1-10. DOI: 10.21767/2471-8203.100034

Turi BC, Codogno JS, Fernandes RA, Monteiro HL. Low levels of physical activity and metabolic syndrome: cross-sectional study in the brazilian public health system. Science & Public Health 2016; 21:1043-1050. DOI: 10.1590/1413-81232015214.23042015

Abboud KY, Reis SK, Martelli ME, Zordão OP, Tannihão F, Souza AZZ, Assalin HB, Guadagnini D, Rocha GZ, Saad MJA, Prada PO. Oral Glutamine Supplementation Reduces Obesity, Pro-Inflammatory Markers, and Improves Insulin Sensitivity in DIOWistar Rats and ReducesWaist Circumference in Overweight and Obese Humans. Nutrients 2019; 11(536):1-16. DOI: 10.3390/nu11030536

Bensimhon DR, Kraus WE, Donahue MP. Obesity and physical activity: A review. American Heart Journal 2006; 151(3):598-603. DOI: 10.1016/j.ahj.2005.03.005

Leung L, Birtwhistle R, Kotecha J, Hannah S, Cuthbertson S. Anti-diabetic and hypoglycaemic effects of momordica charantia (bitter melon): a mini review. Br J nutr. 2009; 102(12):1703-1708. DOI: 10.1017/S0007114509992054.

Rodrigues KAF, Dias CN, Florêncio JC, Vilanova CM, Gonçalves JRS. Phytochemical prospecting and mollusk activity of leaves of Momordica charantia L. Research Notebook 2010; 17:69-76.

Yongmei Y, Xian H, Ebersole B. Bitter melon extract attenuating hepatic steatosis may be mediated by fgf21 and AMPK/sirt1 signaling in mice. Scientific reports 2013; 3(3412):1-8. DOI: 10.1038/srep03142

Estadela D, Oyama LM, Dâmaso AR, Ribeiro EB, Oller Do Nascimento CM. Effect of Palatable Hyperlipidic Diet on Lipid Metabolism of Sedentary and Exercised Rats. Nutrition 2004; 20(2):218-224. DOI: 10.1016/j.nut.2003.10.008

Folch J, Lees M, Sloane-Stanley GH. A simple method forthe isolation and purification of total lipids from animal tissues. J. Biol. Chem. 1957; 226:497-509.

Duarte ACGO, Fonseca DF, Manzoni MSJ, Soave CF, Sene-Fiorese M. Hyperlipidemia and secretory capacity of insulin in rats. Rev. Nutr. 2006; 19(3):341-348.

Kretschmer BD, Schelling P, Beier N, Liebscher C, Treutel S, Kruger N, Scholz HP, Haus A. Modulatory role of food, feeding regime and physical exercise on body weight and insulin resistance. Life science 2005; 76(14):1553-73. DOI: 10.1016/j.lfs.2004.08.028

Wang J, Ryu HK. The effects of momordica charantia on obesity and lipid profiles of mice fed a high-fat diet. Nutrition research and practice 2015; 9:489-495. DOI: 10.4162/nrp.2015.9.5.489

Lin S, Thomas TC, Storlien LH, Huang XF. Development of high fat diet-induced obesity and leptin resistance in mice. Int j obesity 2000; 24:639-46. DOI: 10.1038/sj.ijo.0801209

Ouchi N, Parker JL, Lugus JJ, Walsh K. Adipokines in inflammation and metabolic disease. Nat Rev Immunol. 2011; 11:85-97. DOI: 10.1038 / nri2921

Singh J, Cumming E, Manoharan G, Kalasz H, Adeghate E. Medicinal chemistry of the anti-diabetic effects of Momordica charantia: active constituents and modes of actions. Open Med Chem. J. 2011; 5:70-77. DOI: 10.2174 / 1874104501105010070

Picchii MG, Mattos AM, Barbosa MR, Duarte CP, Gandini MA, Portari GV, Jordão AA. A high-fat diet as a model of fatty liver disease in rats. Acta Brazilian surgical 2011; 26(2):25-30. DOI: 10.1590 / s0102-86502011000800006

Motta VF, Aguila MB, Lacerda CAM. High-intensity interval training beneficial effects in diet- induced obesity in mice: adipose tissue, liver structure, and pancreatic islets. Int. J. Morphol. 2016; 34:684-691. DOI: 10.4067/S0717-95022016000200042

Sridhar MG, Vinayagamoorthi R, Arul SV, Bobby Z, Selvraj N. Bitter gourd (Momordica charantia) improves insulin sensitivity by increasing skeletal muscle insulin stimulated IRS-1 tyrosine phosphorylation in high-fat-fed rats. Br J Nutr. 2008; 99:806–812. DOI: 10.1017 / S000711450783176X

Cheng D, Xu X, Simão T, Boudyguina E, Deng Z, VerHague M, Lee AH, Shelness GS, Weinberg RB, Parks JS. Very low density lipoprotein assembly is required for camp responsive element-binding protein h processing and hepatic apolipoprotein a-iv expression. The american society for biochemistry and molecular biology 2016; 1:1-24. DOI: 10.1074 / jbc.M116.749283

Ciou SY, Hsu CC, Kuo YH, Chao CY. Effect of wild bitter gourd treatment on inflammatory responses in balb/c mice with sepsis. Biomedicine 2014; 4(3):7-13. DOI: 10.7603 / s40681-014-0017-y

Bao B, Chen UG, Zhang L, Na XU, Wang X. Momordica charantia (Bitter Melon) Reduces Obesity-Associated Macrophage and Mast Cell Infiltration as well as Inflammatory Cytokine Expression in Adipose Tissues. PLoS ONE 2013; 8(12): 1-13. DOI: 10.1371 / journal.pone.0084075

Chandru S, Vishwanath P, Devegowda D, Ramasamudra SN, Prashant A, Hathur B. Evaluation of protein kinase cβ and PPAR γ activity in diabetic rats supplemented with momordica charantia. Journal of clinical and diagnostic research 2016; 10:1-4. DOI: 10.7860 / JCDR / 2016 / 18161.7653

Chen HW, Yun WT, Liu HL. Suppressive effects of wild bitter gourd (momordica charantia linn. var. abbreviate ser.) fruit extracts on inflammatory responses in raw264.7 macrophages. J ethnopharmacol 2009; 122:227-233. DOI: 10.1016 / j.jep.2009.01.028

Chao CY, Huang CJ. Bitter gourd (Momordica charantia) extract activates peroxisome proliferator-activated receptors and upregulates the expression of the acyl coa oxidase gene in hepatoma cells. J biomed sci. 2003; 10:782-91. DOI: 10.1159 / 000073966

Cizkova K, Steigerova J, Gursky J, Ehrmann J. Stimulating effect of normal-dosing of fibrates on cell proliferation: word of warning. Lipids in health and disease 2016; 15:1-11. DOI: 10.1186 / s12944-016-0335-z

Zeraati AA, Nazemiano F, Takalloo L, Sahebkar A, Heidari E, Yaghoubi MA. The relationship between serology of hepatitis e virus with liver and kidney function in kidney transplant patient. Excli journal 2016; 15:343-349. DOI: 10.17179 / excli2016-232

Sagor AT, Chowdhury MRH, Tabassum N, Hossain H, Rahman M, Alam MA. Supplementation of fresh ucche (momordica charantia l. var. muricata willd) prevented oxidative stress, fibrosis and hepatic damage in ccl4 treated rats. Bmc complementary & alternative medicine 2015; 15:1- 9. DOI: 10.1186 / s12906-015-0636-1

Righi T, Carvalho, CA, Ribeiro LM, Cunha DNQ, Paiva ACS, Natali AJ, Pereira ET, Lima LM. Alcohol consumption and the influence of physical exercise on enzyme activity of wistar rats. Rev Bras Med Sport 2016; 22(1):40-44. DOI: 10.1590/1517-869220162201141536

Lo HY, Ho TY, Lin C, Li CC, Hsiang CY. Momordica charantia and its novel polypeptide regulate glucose homeostasis in mice via binding to insulin receptor. J. Agric. Food Chem. 2013; 61(10):2461-2468. DOI: 10.1021 / jf3042402

Chou RH, Huang PH, Hsu CY, Chang CC, Leu HB, Huang CC, Chen JW, Lim SJ. Circulating fibroblast growth factor 21 is associated with diastolic dysfunction in heart failure patients with preserved ejection fraction. Scientific Reports 2016; 6:1-10. DOI: 10.1038 / srep33953

Huynh MKQ, Kinyua AW, Yang DJ, Kim KW. Hypothalamic ampk as a regulator of energy homeostasis. Neural plasticity 2016; 16:1-12. DOI: 10.1155/2016/2754078

Tan MJ, Ye JM, Turner N, Hohnen-Behrens C, Ke CQ, Tang CP, Chen T, Weiss HC, Gesing ER, Rowland A, James DE, Ye Y. Antidiabetic activities of triterpenoids isolated from bitter melon associated with the activation of AMPK pathway. ChemBiol. 2008; 5:263–273. DOI: 10.1016 / j.chembiol.2008.01.013


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